Which step is necessary when preparing a single/multidose vial vaccine?

When preparing a single/multidose vial vaccine, shaking the vaccine is often necessary to ensure that the components are properly mixed. Some vaccines contain adjuvants or other ingredients that may settle over time, and gentle shaking helps to evenly distribute these components throughout the vial. This step is crucial for achieving the intended efficacy of the vaccine, as uneven distribution can lead to administering an improper dose or losing the effectiveness of the vaccine.

It's important to note that not all vaccines should be shaken vigorously; the manufacturer’s instructions will specify the appropriate manner of mixing. Proper preparation is essential for the safety and efficacy of the vaccination process.